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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
05/03/2013 |
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Data da última atualização: |
11/03/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BRASILEIRO, A. C. M.; MORGANTE, C. V.; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; PAPPAS, G.; SILVA JUNIOR, O. B. da; MARTINS, A. C. Q.; BERTIOLI, D.; GUIMARAES, P. M. |
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Afiliação: |
ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; CAROLINA VIANNA MORGANTE, CPATSA; SORAYA CRISTINA DE M LEAL BERTIOLI, CENARGEN; ANA CLAUDIA GUERRA DE ARAUJO, CENARGEN; GEORGIOS JOANNIS PAPPAS JUNIOR, CENARGEN; ORZENIL BONFIM DA SILVA JUNIOR, CENARGEN; ABADIO HERMES VIEIRA, CPAF-RO; PATRICIA MESSEMBERG GUIMARAES, CENARGEN. |
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Título: |
Transcriptome analysis in response to gradual water deficit in Arachis wild relatives. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
Peanut (cultivated tetraploid Arachis hypogaea) is an important food legume widely cultivated mainly in Asia, Africa and the Americas although its productivity is limited in drought-prone areas; therefore the development of drought-resistant varieties is a priority. Peanut has a narrow genetic diversity and is reproductively isolated from its wild diploid relatives due to ploidy differences. In contrast to peanut, wild relatives have higher genetic diversity and show adaptation to a range of environments thus constituting a rich source of allele diversity for resistance to biotic and tolerance to abiotic stresses. In this study, the transcriptomes of two wild diploids, A. duranensis and A. magna, representatives of the AA and BB peanut component genomes respectively, were analyzed under gradual water deficit. Two SSH libraries produced from roots of A. magna generated 759 Sanger ESTs with 249 Unigenes, whilst from A. duranensis roots, 380,601 Roche/454 reads resulted in12,792 Unigenes. Forty-six Unigenes were selected as drought-related candidates for validation by RT-qPCR by in silico analysis of all libraries. A total of 18 candidate genes showed significantly different levels of expression in stressed plants, confirming their involvement in drought response. To date, this is the first report on global gene expression profile of wild Arachis species submitted to an abiotic stress. Data produced in this study is a valuable resource for gene identification, characterization of new alleles and development of molecular markers in Arachis. MenosPeanut (cultivated tetraploid Arachis hypogaea) is an important food legume widely cultivated mainly in Asia, Africa and the Americas although its productivity is limited in drought-prone areas; therefore the development of drought-resistant varieties is a priority. Peanut has a narrow genetic diversity and is reproductively isolated from its wild diploid relatives due to ploidy differences. In contrast to peanut, wild relatives have higher genetic diversity and show adaptation to a range of environments thus constituting a rich source of allele diversity for resistance to biotic and tolerance to abiotic stresses. In this study, the transcriptomes of two wild diploids, A. duranensis and A. magna, representatives of the AA and BB peanut component genomes respectively, were analyzed under gradual water deficit. Two SSH libraries produced from roots of A. magna generated 759 Sanger ESTs with 249 Unigenes, whilst from A. duranensis roots, 380,601 Roche/454 reads resulted in12,792 Unigenes. Forty-six Unigenes were selected as drought-related candidates for validation by RT-qPCR by in silico analysis of all libraries. A total of 18 candidate genes showed significantly different levels of expression in stressed plants, confirming their involvement in drought response. To date, this is the first report on global gene expression profile of wild Arachis species submitted to an abiotic stress. Data produced in this study is a valuable resource for gene identification, characterization ... Mostrar Tudo |
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Palavras-Chave: |
Cultura resistente a seca; Drought-prone areas; Melhoramento genético; Peanut; Tolerância à seca. |
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Thesagro: |
Amendoim; Arachis Hypogaea; Melhoramento genético vegetal; Planta oleaginosa. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/77977/1/MORGANTE0002.pdf
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Marc: |
LEADER 02609nam a2200313 a 4500
001 1952220
005 2013-03-11
008 2012 bl uuuu u00u1 u #d
100 1 $aBRASILEIRO, A. C. M.
245 $aTranscriptome analysis in response to gradual water deficit in Arachis wild relatives.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT$c2012
520 $aPeanut (cultivated tetraploid Arachis hypogaea) is an important food legume widely cultivated mainly in Asia, Africa and the Americas although its productivity is limited in drought-prone areas; therefore the development of drought-resistant varieties is a priority. Peanut has a narrow genetic diversity and is reproductively isolated from its wild diploid relatives due to ploidy differences. In contrast to peanut, wild relatives have higher genetic diversity and show adaptation to a range of environments thus constituting a rich source of allele diversity for resistance to biotic and tolerance to abiotic stresses. In this study, the transcriptomes of two wild diploids, A. duranensis and A. magna, representatives of the AA and BB peanut component genomes respectively, were analyzed under gradual water deficit. Two SSH libraries produced from roots of A. magna generated 759 Sanger ESTs with 249 Unigenes, whilst from A. duranensis roots, 380,601 Roche/454 reads resulted in12,792 Unigenes. Forty-six Unigenes were selected as drought-related candidates for validation by RT-qPCR by in silico analysis of all libraries. A total of 18 candidate genes showed significantly different levels of expression in stressed plants, confirming their involvement in drought response. To date, this is the first report on global gene expression profile of wild Arachis species submitted to an abiotic stress. Data produced in this study is a valuable resource for gene identification, characterization of new alleles and development of molecular markers in Arachis.
650 $aAmendoim
650 $aArachis Hypogaea
650 $aMelhoramento genético vegetal
650 $aPlanta oleaginosa
653 $aCultura resistente a seca
653 $aDrought-prone areas
653 $aMelhoramento genético
653 $aPeanut
653 $aTolerância à seca
700 1 $aMORGANTE, C. V.
700 1 $aBERTIOLI, S. C. de M. L.
700 1 $aARAUJO, A. C. G. de
700 1 $aPAPPAS, G.
700 1 $aSILVA JUNIOR, O. B. da
700 1 $aMARTINS, A. C. Q.
700 1 $aBERTIOLI, D.
700 1 $aGUIMARAES, P. M.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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| Registros recuperados : 18 | |
| 3. |  | SANSALONI, C. P.; MAMANI, E. M.; PAPPAS, G. J.; GRATTAPAGLIA, D. Análise populacional e mapeamento genético de microssatélites tetra e pentanucleotídeos em espécies de Eucalyptus. In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 158.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | CASTRO, A. P.; PAPPAS, G.; QUIRINO, B. F.; KRUGER, R. H. In silico analysis to compare the 18S rRNA gene libraries and estimate species richeness whitin Biome Cerrado. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 6. | | PAPPAS, M. R.; FARIA, D. A.; PURIM, S. G.; PAPPAS, G. J.; GRATTAPAGLIA, D. Sequencing the eucalyptus grandis genome: a microsatellite survey shows maintenance of heterozygosity in the target, Partially Inbred. genome. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego, 2008, W196.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 7. | | GRATTAPAGLIA, D.; NOVAES, E.; PAPPAS, G. J.; PASQUALI, G.; KIRST, M. Single feature polymorphism discovery and validation in Eucalyptus by pseudo-testcross inheritance and mapping. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego, 2008, W172.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | NEVES, L. G.; FARIA, D. A.; PAPPAS, G. J. J. R.; PASQUALI, G.; GRATTAPAGLIA, D. Diversidade nucleotídica e mapeamento de genes candidatos em Eucalyptus spp. In : ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 12., 2007, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. p. 170.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | QUIRINO, B. F.; BARRETO, C. C.; PAPPAS, G. J.; ZEGLE, K.; KRAMPIS, K.; KRUGER, R. H. Genomes and post-genome technology. In: FALKOW, S.; ROSENBERG, E.; SCHLEIFER, K-H.; STACKEBRANDT, E.; DWORKIN, M. (Ed.). The prokaryotes. Berlin: Springer-Verlag, 2013.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | LOURENÇO, R. T.; PAPPAS, G. J.; CUNHA, A. F.; PEREIRA, G. A. G.; GRATTAPAGLIA, D. Estrutura genômica de três megabases de dna genômico "shotgun" de eucalyptus: conteúdo nucleotídico, seouências repetitivas e genes. ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENETICOS E BIOTECNOLOGIA, 8., 2003, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2003. p. 74| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 11. | | PAPPAS, G. J.; BROMMONSCHENKEL, S. H.; PAPPAS, M. R.; FARIA, D. A.; SÁ, S. V.; KAISER, O.; GRATTAPAGLIA, D. Individual and pooled eucalyptus BAC clone sequencing and assembly by high throughput pyrosequencing. In: THE INTERNATIONAL CONFERENCE ON THE STATUS OF PLANT & ANIMAL GENOME RESEARCH, 16., 2008, San Diego. Final abstracts guide. San Diego, 2008, W175.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 12. | | PEREIRA, J. L.; FRANCO, O. L.; PAULINO, L.; PAPPAS, G. J.; BLOCH JUNIOR, C.; GRATTAPAGLIA, D.; NORONHA, E. F. Comparative xylem proteome of Eucalyptus grandis and Eucalyptus globulus. In: ANNUAL MEETING OF SBBQ, 37.; CONGRESS OF THE PABMB, 11., 2008, Águas de Lindóia, SP. Abstracts... Águas de Lindóia: SBBq, 2008. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 13. | | BEVITORI, R.; MEYERS, B.; NAKANO, M.; JAIN, G.; PAPPAS, G. J.; FRAGOSO, R.; TRISTAN, I.; SÁ, M. F. G. de. Transcriptome profiling of Magnaporthe grisea-infected rice using next-generation sequencing. In: INTERNATIONAL PLANT MOLECULAR BIOLOGY, 9., 2009, St. Louis. Conference program. St. Louis: International Society for Plant Molecular Biology; University of Missouri, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 14. | | PAPPAS, M.; PEREIRA, W.; INGLIS, P. W.; PADILHA, J.; KILIAN, A.; STAPE, J.; PAPPAS, G.; GRATTAPAGLIA, D. Differential patterns of genome-wide methylation within and between Eucalyptus clones across variable environments. In: PLANT AND ANIMAL GENOME CONFERENCE, 25., 2017, San Diego. [Abstracts...]. San Diego, CA: [s.n.], 2017. W339.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 15. | | PAPPAS, M. C. R.; PAPPAS, G. J.; BROMMONSCHENKEL, S. H.; FARIA, D. A.; SÁ, S. V.; KAISER, O.; GRATTAPAGLIA, D. Eucalyptus BAC clones sequencing and assembly using 454 technology. In: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Resumos... Salvador: SBG, 2008. p. 321. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 16. | | GRATTAPAGLIA, D.; ALFENAS, A.; COELHO, A.; PAPPAS, G.; PASQUALI, G.; PEREIRA, G.; COLODETTE, J.; GLOMIDE, J. L.; BUENO, J.; CASCARDO, J.; BRONDANI, R.; BROMMONSCHENKEL, S. Building resources for molecular breeding of Eucalyptus: the genolyptus project. In: REUNIÃO ANUAL: SOCIEDADE BRASILEIRA DE BIOQUÍMICA E BIOLOGIA MOLECULAR, 34., 2005, Águas de Lindóia. Programas e resumos... São Paulo: Sociedade Brasileira de Bioquímica e Biologia Molecular, 2005.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 17. | | BRASILEIRO, A. C. M.; MORGANTE, C. V.; BERTIOLI, S. C. de M. L.; ARAUJO, A. C. G. de; PAPPAS, G.; SILVA JUNIOR, O. B. da; MARTINS, A. C. Q.; BERTIOLI, D.; GUIMARAES, P. M. Transcriptome analysis in response to gradual water deficit in Arachis wild relatives. In: INTERNATIONAL CONFERENCE ON LEGUME GENETICS AND GENOMICS, 6., 2012, Hyderabad, India. Programe & abstract book... Hyderabad: ICRISAT, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Semiárido. |
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| 18. | | BRUSKIEWICH, R.; SENGER, M.; DAVENPORT, G.; RUIZ, M.; ROUARD, M.; HAZEKAMP, T.; TAKEYA, M.; DOI, K.; SATOH, K.; COSTA, M.; SIMON, R.; BALAJI, J.; AKINTUNDE, A.; MAULEON, R.; WANCHANA, S.; SHAH, T.; ANACLETO, M.; PORTUGAL, A.; ULAT, V. J.; THONGJUEA, S.; BRAAK, K.; RITTER, S.; DEREEPER, M.; SKOFIC, M.; ROJAS, E.; MARTINS, N.; PAPPAS, G.; ALAMBAN, R.; ALMODIEL, R.; BARBOZA, L. H.; DETRAS, J.; MANANSALA, K.; MENDOZA, M. J.; MORALES, J.; PERALTA, B.; VALERIO, R.; ZHANG, Y.; GREGORIO, S.; HERMOCILLA, J.; ECHAVEZ, M.; YAP, J. M.; FARMER, A.; SCHILTZ, G.; LEE, J.; CASSTEVENS, T.; JAISWAL, P.; MEINTJES, A.; WILKINSON, M.; GOOD, B.; WAGNER, J.; MORRIS, J.; MARSHALL, D.; COLLINS, A.; KIKUCHI, S.; METZ, T.; MCLAREN, G.; HINTUM, T. van. The generation challenge programme platform: semantic standards and workbench for crop science. International Journal of Plant Genomics, v.2008, 6 p., 2008. doi:10.1155/2008/369601.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - B |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 18 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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